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dc.contributor.authorRebers, Lisa-
dc.contributor.authorReichsöllner, Raffael-
dc.contributor.authorRegett, Sophia-
dc.contributor.authorTovar, Günter E. M.-
dc.contributor.authorBorchers, Kirsten-
dc.contributor.authorBaudis, Stefan-
dc.contributor.authorSouthan, Alexander-
dc.description.abstractGelatin methacryloyl (GM) hydrogels have been investigated for almost 20 years, especially for biomedical applications. Recently, strengthening effects of a sequential cross-linking procedure, whereby GM hydrogel precursor solutions are cooled before chemical cross-linking, were reported. It was hypothesized that physical and enhanced chemical cross-linking of the GM hydrogels contribute to the observed strengthening effects. However, a detailed investigation is missing so far. In this contribution, we aimed to reveal the impact of physical and chemical cross-linking on strengthening of sequentially cross-linked GM and gelatin methacryloyl acetyl (GMA) hydrogels. We investigated physical and chemical cross-linking of three different GM(A) derivatives (GM10, GM2A8 and GM2), which provided systematically varied ratios of side-group modifications. GM10 contained the highest methacryloylation degree (DM), reducing its ability to cross-link physically. GM2 had the lowest DM and showed physical cross-linking. The total modification degree, determining the physical cross-linking ability, of GM2A8 was comparable to that of GM10, but the chemical cross-linking ability was comparable to GM2. At first, we measured the double bond conversion (DBC) kinetics during chemical GM(A) cross-linking quantitatively in real-time via near infrared spectroscopy-photorheology and showed that the DBC decreased due to sequential cross-linking. Furthermore, results of circular dichroism spectroscopy and differential scanning calorimetry indicated gelation and conformation changes, which increased storage moduli of all GM(A) hydrogels due to sequential cross-linking. The data suggested that the total cross-link density determines hydrogel stiffness, regardless of the physical or chemical nature of the cross-links.en
dc.titleDifferentiation of physical and chemical cross-linking in gelatin methacryloyl hydrogelsen
ubs.fakultaetEnergie-, Verfahrens- und Biotechnikde
ubs.fakultaetExterne wissenschaftliche Einrichtungende
ubs.fakultaetFakultätsübergreifend / Sonstige Einrichtungde
ubs.institutInstitut für Grenzflächenverfahrenstechnik und Plasmatechnologiede
ubs.institutFraunhofer Institut für Grenzflächen- und Bioverfahrenstechnik (IGB)de
ubs.institutFakultätsübergreifend / Sonstige Einrichtungde
ubs.publikation.sourceScientific reports 11 (2021), No. 3256de
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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