Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
http://dx.doi.org/10.18419/opus-1966
Langanzeige der Metadaten
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Klünder, Irene | de |
dc.contributor.author | Hülser, Dieter F. | de |
dc.date.accessioned | 2011-12-05 | de |
dc.date.accessioned | 2016-03-31T07:53:06Z | - |
dc.date.available | 2011-12-05 | de |
dc.date.available | 2016-03-31T07:53:06Z | - |
dc.date.issued | 1991 | de |
dc.identifier.other | 36842832X | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-69045 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/1983 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-1966 | - |
dc.description.abstract | In these studies, we demonstrate that the threedimensional arrangement of the cells in spheroids influences the β-galactosidase activity of transfected ltk--cells. In contrast to cells grown in monolayer culture cells growing as spheroids lost their β-galactosidase activity within few days. The reduction of β-galactosidase activity in cells grown as spheroids was not caused by a lost of the lacZ-gene as can be seen from the recovery of the β-galactosidase production when cells were retransferred in monolayer culture. As we showed in cells cultivated as monolayer, β-galactosidase activity seems to be not impaired by gradients of nutrient supply or cell cycle. These measurements are confirmed by histological sections, where the highest β-galactosidase activity was found in the viable center of the spheroids. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Zellkultur , Galactosidase <beta-> | de |
dc.subject.ddc | 570 | de |
dc.title | β-galactosidase production in two- and threedimensionally cultivated cell cultures | en |
dc.type | conferenceObject | de |
ubs.fakultaet | Fakultät Energie-, Verfahrens- und Biotechnik | de |
ubs.institut | Institut für Biomaterialien und biomolekulare Systeme | de |
ubs.opusid | 6904 | de |
ubs.publikation.source | Reuß, Matthias (Hrsg.): Biochemical engineering - Stuttgart : proceedings of the 2. International Symposium on Biochemical Engineering which was held at the Univ. of Stuttgart from March 5 to 7, 1990. Stuttgart : G. Fischer, 1991. - ISBN 3-437-30662-6, S. 224-227 | de |
ubs.publikation.typ | Konferenzbeitrag | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
huel74.pdf | 626,24 kB | Adobe PDF | Öffnen/Anzeigen |
Alle Ressourcen in diesem Repositorium sind urheberrechtlich geschützt.