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dc.contributor.authorFaridian, Ahmadde
dc.contributor.authorPedrini, Giancarlode
dc.contributor.authorOsten, Wolfgangde
dc.date.accessioned2014-04-16de
dc.date.accessioned2016-03-31T08:17:22Z-
dc.date.available2014-04-16de
dc.date.available2016-03-31T08:17:22Z-
dc.date.issued2014de
dc.identifier.other404268633de
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:bsz:93-opus-90892de
dc.identifier.urihttp://elib.uni-stuttgart.de/handle/11682/4584-
dc.identifier.urihttp://dx.doi.org/10.18419/opus-4567-
dc.description.abstractScattering and absorption belong to the major problems in imaging the internal layers of a biological specimen. Due to the structural inhomogeneity of the specimen, the distribution of the structures in the upper layers of a given internal structure of interest is different from the lower layers that may result in different interception of scattered light, falling into the angular aperture of the microscope objective, from the object in each imaging view. Therefore, different spatial frequencies of the scattered light can be acquired from different (top and bottom) views. We have arranged an opposed-view dark-field digital holographic microscope (DHM) to collect the scattered light concurrently from both views with the aim to increase the contrast of internal structures and improve the signal-to-noise ratio. Implementing a DHM system gives the possibility to implement digital refocusing process and obtain multilayer images from each side without a depth scan of the object. The method is explained and the results are presented exemplary for a Drosophila embryo.en
dc.language.isoende
dc.rightsinfo:eu-repo/semantics/openAccessde
dc.subject.classificationMikroskopie , Holographie , Bildverarbeitungde
dc.subject.ddc530de
dc.titleOpposed-view dark-field digital holographic microscopyen
dc.typearticlede
dc.date.updated2014-05-13de
ubs.fakultaetFakultät Konstruktions-, Produktions- und Fahrzeugtechnikde
ubs.fakultaetInterfakultäre Einrichtungende
ubs.institutInstitut für Technische Optikde
ubs.institutStuttgart Research Centre of Photonic Engineering (SCoPE)de
ubs.opusid9089de
ubs.publikation.sourceBiomedical optics express 5 (2014), S. 728-736. URL http://dx.doi.org/10.1364/BOE.5.000728de
ubs.publikation.typZeitschriftenartikelde
Enthalten in den Sammlungen:07 Fakultät Konstruktions-, Produktions- und Fahrzeugtechnik

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