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DC Field | Value | Language |
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dc.contributor.author | Pieper, Dietmar H. | de |
dc.contributor.author | Stadler-Fritzsche, Karin | de |
dc.contributor.author | Knackmuss, Hans-Joachim | de |
dc.contributor.author | Engesser, Karl-Heinrich | de |
dc.contributor.author | Bruce, Neil C. | de |
dc.contributor.author | Cain, Ronald B. | de |
dc.date.accessioned | 2012-08-22 | de |
dc.date.accessioned | 2016-03-31T11:44:55Z | - |
dc.date.available | 2012-08-22 | de |
dc.date.available | 2016-03-31T11:44:55Z | - |
dc.date.issued | 1990 | de |
dc.identifier.other | 371054478 | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76458 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/8033 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-8016 | - |
dc.description.abstract | 4-Carboxymethyl-4-methylbut-2-en-4-olide (4-methyl-2-enelactone) isomerase, transforming 4-methyl-2-enelactone to 3-methyl-2-enelactone, was purified from a derivative strain of Pseudomonas sp. B13, named B13 FR1, carrying the plasmid pFRC2OP. This plasmid contained the isomerase gene cloned from Alcaligenes eutrophus JMP 134, which uses 4-methyl-2-enelactone as a carbon source. The enzyme consists of a single peptide chain of Mr 40,000 as judged by SDS/PAGE. In addition to 4-methyl-2-enelactone, the putative reaction intermediate, 1-methyl-3,7-dioxo-2,6-dioxy-bicyclo[3.3.0]octane (1-methylbislactone), was a substrate for the enzyme, but kinetic data presented did not favour its role as a reaction intermediate. Isomeric methyl-substituted 4-carboxymethylbut-2-en-4-olides were neither substrates nor inhibitors. Possible reaction mechanisms are discussed. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Alcaligenes eutrophus , Brenzcatechin | de |
dc.subject.ddc | 570 | de |
dc.title | Purification and characterization of 4-methylmuconolactone methylisomerase, a novel enzyme of the modified 3-oxoadipate pathway in the gram-negative bacterium Alcaligenes eutrophus JMP 134 | en |
dc.type | article | de |
ubs.fakultaet | Fakultätsübergreifend / Sonstige Einrichtung | de |
ubs.fakultaet | Fakultät Energie-, Verfahrens- und Biotechnik | de |
ubs.institut | Sonstige Einrichtung | de |
ubs.institut | Institut für Mikrobiologie | de |
ubs.opusid | 7645 | de |
ubs.publikation.source | Biochemical journal 271 (1990), S. 529-534. URL http://www.biochemj.org/bj/271/0529/2710529.pdf | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Appears in Collections: | 15 Fakultätsübergreifend / Sonstige Einrichtung |
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