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http://dx.doi.org/10.18419/opus-813
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DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Maurer, Steffen Christian | de |
dc.contributor.author | Schulze, Holger | de |
dc.contributor.author | Schmid, Rolf D. | de |
dc.contributor.author | Urlacher, Vlada B. | de |
dc.date.accessioned | 2006-06-01 | de |
dc.date.accessioned | 2016-03-31T07:46:42Z | - |
dc.date.available | 2006-06-01 | de |
dc.date.available | 2016-03-31T07:46:42Z | - |
dc.date.issued | 2003 | de |
dc.identifier.other | 263636542 | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26527 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/830 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-813 | - |
dc.description.abstract | Cytochrome P450 monooxygenases are potentially a very useful class of hydroxylation catalysts; they are able to introduce oxygen at activated and non-activated carbon-hydrogen bonds and thus lead to regio- and/or stereochemically pure compounds. However, this potential is lowered by their intrinsic low activity and inherent instability. P450-catalysed biotransformations require a constant supply of NAD(P)H, making the process an expensive one. To render these catalysts more suitable for industrial biocatalysis, the immobilisation of P450 BM-3 (CYP 102A1) from Bacillus megaterium in a sol-gel matrix was combined with a cofactor recycling system based on NADPƒy-dependent formate dehydrogenase (EC 1.2.1.2) from Pseudomonas sp. 101 and tested for practical applicability. This approach was used for the conversion of £]-ionone, octane and naphthalene to the respective hydroxy-compounds with DMSO as cosolvent using sol-gel immobilised P450 BM-3 mutants. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Cytochrom P-450 , Monooxygenasen , Biokatalyse , Biokonversion , Immobilisierung , Biotransformation , Hydroxylierung | de |
dc.subject.ddc | 540 | de |
dc.subject.other | cofactor recycling , fine chemical synthesis , sol-gel , cytochrome P450 BM-3 | en |
dc.title | Immobilisation of P450 BM-3 and an NADP+ cofactor recycling system : towards a technical application of heme-containing monooxygenases in fine chemical synthesis | en |
dc.type | preprint | de |
dc.date.updated | 2015-12-09 | de |
ubs.fakultaet | Fakultät Chemie | de |
ubs.institut | Institut für Technische Biochemie | de |
ubs.opusid | 2652 | de |
ubs.publikation.source | Advanced synthesis & catalysis 345 (2003), S. 802-810. URL http://dx.doi.org./10.1002/adsc.200303021 | de |
ubs.publikation.typ | Preprint | de |
Enthalten in den Sammlungen: | 03 Fakultät Chemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Maurer_2003.pdf | 578,4 kB | Adobe PDF | Öffnen/Anzeigen |
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