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http://dx.doi.org/10.18419/opus-820
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DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Urlacher, Vlada B. | de |
dc.contributor.author | Makhsumkhanov, Akhmadjan | de |
dc.contributor.author | Schmid, Rolf D. | de |
dc.date.accessioned | 2006-06-01 | de |
dc.date.accessioned | 2016-03-31T07:46:43Z | - |
dc.date.available | 2006-06-01 | de |
dc.date.available | 2016-03-31T07:46:43Z | - |
dc.date.issued | 2005 | de |
dc.identifier.other | 263638626 | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26603 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/837 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-820 | - |
dc.description.abstract | Wild type cytochrome P450 monooxygenase from Bacillus megaterium (P450 BM-3) has low activity for the hydroxylation of beta-ionone (>1 min-1). Substitution of phenylalanine by valine at position 87 increased the beta-ionone hydroxylation activity up to 100-fold (115 min-1). For further activity improvement methods of site-directed and random mutagenesis were applied. The R47L Y51F F87V mutant, designed by site-directed mutagenesis and the A74E F87V P386S mutant, obtained after two rounds of error-prone PCR, exhibit an increase in activity up to 300-fold compared to the wild type enzyme. All mutants converted -ionone regioselectively to 4-hydroxy-beta-ionone. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Cytochrom P-450 , Monooxygenasen , Biokatalyse , Biokonversion , Biotransformation , Hydroxylierung , Mutagenese , Genbibliothek | de |
dc.subject.ddc | 540 | de |
dc.subject.other | P450 BM-3 , beta-ionone , regioselectivity , mutagenesis , gene library | en |
dc.title | Biotransformation of ionones by engineered cytochrome P450 BM-3 | en |
dc.type | preprint | de |
dc.date.updated | 2015-12-10 | de |
ubs.fakultaet | Fakultät Chemie | de |
ubs.institut | Institut für Technische Biochemie | de |
ubs.opusid | 2660 | de |
ubs.publikation.source | Applied microbiology & biotechnology 70 (2006), S. 53-59. URL http://dx.doi.org./10.1007/s00253-005-0028-4 | de |
ubs.publikation.typ | Preprint | de |
Enthalten in den Sammlungen: | 03 Fakultät Chemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Urlacher_2005.pdf | 113,96 kB | Adobe PDF | Öffnen/Anzeigen |
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