Interaction of nitrate assimilation and photorespiration at elevated CO2

dc.contributor.authorKrämer, Konrad
dc.contributor.authorBrock, Judith
dc.contributor.authorHeyer, Arnd G.
dc.date.accessioned2024-04-24T09:18:31Z
dc.date.available2024-04-24T09:18:31Z
dc.date.issued2022de
dc.date.updated2023-11-14T01:29:48Z
dc.description.abstractIt has been shown repeatedly that exposure to elevated atmospheric CO2 causes an increased C/N ratio of plant biomass that could result from either increased carbon or - in relation to C acquisition - reduced nitrogen assimilation. Possible reasons for diminished nitrogen assimilation are controversial, but an impact of reduced photorespiration at elevated CO2 has frequently been implied. Using a mutant defective in peroxisomal hydroxy-pyruvate reductase (hpr1-1) that is hampered in photorespiratory turnover, we show that indeed, photorespiration stimulates the glutamine-synthetase 2 (GS) / glutamine-oxoglutarate-aminotransferase (GOGAT) cycle, which channels ammonia into amino acid synthesis. However, mathematical flux simulations demonstrated that nitrate assimilation was not reduced at elevated CO2, pointing to a dilution of nitrogen containing compounds by assimilated carbon at elevated CO2. The massive growth reduction in the hpr1-1 mutant does not appear to result from nitrogen starvation. Model simulations yield evidence for a loss of cellular energy that is consumed in supporting high flux through the GS/GOGAT cycle that results from inefficient removal of photorespiratory intermediates. This causes a futile cycling of glycolate and hydroxy-pyruvate. In addition to that, accumulation of serine and glycine as well as carboxylates in the mutant creates a metabolic imbalance that could contribute to growth reduction.en
dc.identifier.issn1664-462X
dc.identifier.other1887319077
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-142822de
dc.identifier.urihttp://elib.uni-stuttgart.de/handle/11682/14282
dc.identifier.urihttp://dx.doi.org/10.18419/opus-14263
dc.language.isoende
dc.relation.uridoi:10.3389/fpls.2022.897924de
dc.rightsinfo:eu-repo/semantics/openAccessde
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de
dc.subject.ddc570de
dc.titleInteraction of nitrate assimilation and photorespiration at elevated CO2en
dc.typearticlede
ubs.fakultaetEnergie-, Verfahrens- und Biotechnikde
ubs.institutInstitut für Biomaterialien und biomolekulare Systemede
ubs.publikation.seiten14de
ubs.publikation.sourceFrontiers in plant science 13 (2022), No. 897924de
ubs.publikation.typZeitschriftenartikelde

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