A rapid method for the determination of absolute particle sizes in a flow cytometer

Brenner, Joachim; Hülser, Dieter F.

A rapid method for the determination of absolute particle sizes in a flow cytometer

dc.contributor.author	Brenner, Joachim	de
dc.contributor.author	Hülser, Dieter F.	de
dc.date.accessioned	2011-11-21	de
dc.date.accessioned	2016-03-31T07:53:02Z
dc.date.available	2011-11-21	de
dc.date.available	2016-03-31T07:53:02Z
dc.date.issued	1991	de
dc.description.abstract	The most precise determination of a cell-size distribution can be expected by registering the signal of a three-dimensional size (particle volume). This is best performed in a flow cytometer with an integrated volume measurement according to the Coulter-principle.This electrical measurement is very fast and is more precise than the forward-scatter method which is used alternativly in flow cytometry. The mean size of particles which has been determined by the Coulter-principle is mostly given by arbitrary units. We, therefore, present here a simple and fast calibration method which allows the transformation of arbitrary to absolute values. In addition we compared these data with one- and two-dimensional measurements under the microscope and an image-processing-system, respectively.	en
dc.identifier.other	368374432	de
dc.identifier.uri	http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68913	de
dc.identifier.uri	http://elib.uni-stuttgart.de/handle/11682/1968
dc.identifier.uri	http://dx.doi.org/10.18419/opus-1951
dc.language.iso	en	de
dc.rights	info:eu-repo/semantics/openAccess	de
dc.subject.classification	Durchflusscytometrie , Zellkultur	de
dc.subject.ddc	570	de
dc.title	A rapid method for the determination of absolute particle sizes in a flow cytometer	en
dc.type	bookPart	de
ubs.fakultaet	Fakultät Energie-, Verfahrens- und Biotechnik	de
ubs.institut	Institut für Biomaterialien und biomolekulare Systeme	de
ubs.opusid	6891	de
ubs.publikation.source	Schütt, Wolfgang (Hrsg.): Physical characterization of biological cells. Berlin : Verl. Gesundheit, 1991. - ISBN 3-333-00632-4, S. 131-146	de
ubs.publikation.typ	Buchbeitrag	de

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