Klünder, IreneHülser, Dieter F.2011-12-052016-03-312011-12-052016-03-31199136842832Xhttp://nbn-resolving.de/urn:nbn:de:bsz:93-opus-69045http://elib.uni-stuttgart.de/handle/11682/1983http://dx.doi.org/10.18419/opus-1966In these studies, we demonstrate that the threedimensional arrangement of the cells in spheroids influences the β-galactosidase activity of transfected ltk--cells. In contrast to cells grown in monolayer culture cells growing as spheroids lost their β-galactosidase activity within few days. The reduction of β-galactosidase activity in cells grown as spheroids was not caused by a lost of the lacZ-gene as can be seen from the recovery of the β-galactosidase production when cells were retransferred in monolayer culture. As we showed in cells cultivated as monolayer, β-galactosidase activity seems to be not impaired by gradients of nutrient supply or cell cycle. These measurements are confirmed by histological sections, where the highest β-galactosidase activity was found in the viable center of the spheroids.eninfo:eu-repo/semantics/openAccessZellkultur , Galactosidase <beta->570β-galactosidase production in two- and threedimensionally cultivated cell culturesconferenceObject