Layh, NormanStolz, AndreasFörster, SiegfriedEffenberger, FranzKnackmuss, Hans-Joachim2009-08-282016-03-312009-08-282016-03-311992316208698http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-44371http://elib.uni-stuttgart.de/handle/11682/1831http://dx.doi.org/10.18419/opus-1814Bacteria were enriched from soil samples, using benzylcyanide, α-methyl-, α-ethyl- or α-methoxybenzyl-cyanide as the sole source of nitrogen. All isolated strains belonged to the genus Pseudomonas. Resting cells of the isolates hydrolysed O-acetylmandelonitrile to O-acetylmandelic acid, O-acetylmandelic acid amide and mandelic acid. From racemic O-acetylmandelonitrile all isolates preferentially formed R(–)-acetylmandelic acid ( = d-acetylmandelic acid). The enantioselective hydrolysis of O-acetylmandelonitrile could also be demonstrated in vitro. Crude extracts did not hydrolyse O-acetylmandelic acid amide indicating an enantioselective nitrilase rather than a nitrile hydratase/amidase system.eninfo:eu-repo/semantics/openAccessNitrile , Pseudomonas , Enantioselektivität , Mandelsäurederivate570article2010-09-30