Engesser, Karl-HeinrichSchulte, P.2012-08-222016-03-312012-08-222016-03-311989371096405http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76505http://elib.uni-stuttgart.de/handle/11682/2027http://dx.doi.org/10.18419/opus-2010Pseudomonas putida strain CLB 250 (DSM 5232) utilized 2-bromo-, 2-chloro- and 2-fluorobenzoate as sole source of carbon and energy. Degradation is suggested to be initiated by a dioxygenase liberating halide in the first catabolic step. After decarboxylation and rearomatization catechol is produced as a central metabolite which is degraded via the ortho-pathway. After inhibition of ring cleavage activities with 3-chlorocatechol, 2-chlorobenzoate was transformed to catechol in nearly stoichiometric amounts. Other ortho-substituted benzoates like anthranilate and 2-methoxybenzoate seem to be metabolized via the same route.eninfo:eu-repo/semantics/openAccessPseudomonas , Degradation , Benzoate570article