Hülser, Dieter F.Webb, Dennis J.2011-10-242016-03-312011-10-242016-03-311973357176170http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-67910http://elib.uni-stuttgart.de/handle/11682/7782http://dx.doi.org/10.18419/opus-7765The tip potential of Ling-Gerard glass microelectrodes changes upon insertion into cells and thus impedes the determination of the actual membrane potential. The lower the membrane potential of a cell, the larger will be the error due to this tip potential. However, as is demonstrated, a relationship exists between the tip potential of the electrode and the measured potential difference, which allows the determination of the membrane potential of a particular cell type by linear regression. This method showed that resting lymphocytes had no membrane potential, whereas for the slime mould Dictyostelitium discoideum a membrane potential of about -9 mV could be calculated.eninfo:eu-repo/semantics/openAccessBiomembran , Mikroelektrode570article