(+)-4-Carboxymethyl-2,4-dimethylbut-2-en-4-olide as dead-end metabolite of 2,4-dimethylphenoxyacetic acid or 2,4-dimethylphenol by alcaligenes eutrophus JMP 134

Pieper, Dietmar H.Engesser, Karl-HeinrichKnackmuss, Hans-Joachim2012-08-152016-03-312012-08-152016-03-311990371010942http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76288http://elib.uni-stuttgart.de/handle/11682/2020http://dx.doi.org/10.18419/opus-20032,4-Dimethylphenoxyacetic acid and 2,4-dimethylphenol are not growth substrates for Alcaligenes eutrophus JMP 134 although being cooxidized by 2,4-dichlorophenoxyacetate grown cells. None of the relevant catabolic pathways were induced by the dimethylphenoxyacetate, 3,5-Dimethylcatechol is not subject to metacleavage. The alternative ortho-eleavage is also unproductive and gives rise to (+)-4-carboxymethyl-2,4-dimethylbut-2-en-4-olide as a dead-end metabolite. High yields of this metabolite were obtained with the mutant Alcaligenes eutrophys JMP 134-1 which constitutively expresses the genes of 2,4-dichlorophenoxyacetic acid metabolism.eninfo:eu-repo/semantics/openAccessAlcaligenes eutrophus , Chloraromaten , Dimethylphenole , Degradation570(+)-4-Carboxymethyl-2,4-dimethylbut-2-en-4-olide as dead-end metabolite of 2,4-dimethylphenoxyacetic acid or 2,4-dimethylphenol by alcaligenes eutrophus JMP 134article