Brümmer, FranzZempel, GüntherBühle, PeterStein, Johannes-ChristophHülser, Dieter F.2011-11-212016-03-312011-11-212016-03-311991363846573http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68876http://elib.uni-stuttgart.de/handle/11682/1963http://dx.doi.org/10.18419/opus-1946All-trans retinoic acid (RA), which was recently identified as a morphogen, affects gap junctional permeability in a dose- and time-dependent manner. In five different established mammalian cell lines (FL, BRL, BICR/M1Rk, HEL37, BT5C1) 100 μmol/liter RA reduced Lucifer yellow spreading within 30 min to 20-50% of the control. Ionic coupling, however, remained almost unaffected under the same conditions. Freezefractured membranes of untreated and RA-treated cells were similar with regard to frequency and sizes of gap junction plaques. With concentrations of less than 10 μmol/liter RA the dye spreading increased significantly in the human amniotic cell line FL, pointing to a possible modulatory effect of RA on junctional communication.eninfo:eu-repo/semantics/openAccessZellkultur , Gap junction , Retinoide570article2011-11-22