Eckert, ReinerDunina-Barkovskaja, AntoninaHülser, Dieter F.2011-12-052016-03-312011-12-052016-03-311993363923489http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-69134http://elib.uni-stuttgart.de/handle/11682/1975http://dx.doi.org/10.18419/opus-1958HeLa cells seem not to be junctionally coupled when probed with techniques such as Lucifer yellow spreading and/or ionic coupling measured with three inserted microelectrodes. When investigated with double whole-cell patch-clamp measurements, HeLa cells in monolayer cultures were electrically coupled in 39% of the cases with very low transjunctional conductances (average one to five open channels). These gapjunction channels had a single-channel conductance γ=26±6 pS and were voltage-gated with an equivalent gating charge ζ=3.1±1.5 for a voltage of half-maximal inactivation Uo=49±10 mV. The voltage-dependent component represents only 31±8% of the total junctional conductance. The voltage-insensitive conductance is characterized by a residual open probability po(∞)=0.34±0.12, which corresponds to a ratio Gmin/Gmax=0.50±0.12. Dissociation of monolayer cells into cell pairs yielded about 58% coupled cell pairs with no notably altered single-channel properties.eninfo:eu-repo/semantics/openAccessZellkommunikation , Gap junction , HeLa-Zelle570article2011-12-05