Engesser, Karl-HeinrichRubio, Miguel AngelKnackmuss, Hans-Joachim2012-08-222016-03-312012-08-222016-03-311990371052718http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76440http://elib.uni-stuttgart.de/handle/11682/2025http://dx.doi.org/10.18419/opus-2008Sixteen bacterial strains capable of degrading alkylbenzenes and alkylphenols were directly isolated from soil and water. The degradation pathways are discussed. Alkylcatechols are almost exclusively cleaved via meta-ring fission. Meta-cleavage of 3-trifluoromethyl-(TFM)-catechol was observed with all strains at different rates although the reaction rates compared to catechol as a substrate varied considerably. All 2-hydroxy-6-oxohepta-2,4-dienoic acid hydrolases investigated showed strong binding of 7,7,7-trifluoro-2-hydroxy-6-oxohepta-2,4-dienoic acid, the ring fission product of 3-TFM-catechol. Turnover rates, however, were negligible indicating this compound to be a general dead-end metabolite during metabolism of TFM-substituted compounds via meta-cleavage pathways.eninfo:eu-repo/semantics/openAccessPseudomonas , Stoffwechsel , Aromaten570article