Osipov, A. P.Gapanova, N. K.Egorov, A. M.Berezin, I. V.Pertschi, Ottmar (Übersetzer)2008-11-142016-03-312008-11-142016-03-311991313529361http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-37497http://elib.uni-stuttgart.de/handle/11682/6252http://dx.doi.org/10.18419/opus-6235The activity of alk. phosphatase is detd. by incubation of a sample with NADP (I) in a buffer medium with subsequent spectrophotometric recording of the hydrolysis product in an enzymic regeneration system of a cofactor-contg. alk. dehydrogenase and two substrates, one of which is EtOH. The sensitivity of the detn. is increased and a rapid and simplified detn. is ensured by incubation of the sample and recording of the hydrolysis product catalyzed by alk. phosphatase in a pH 8,5 - 9,1 tris-HCl buffer; By using I in reduced form at 10-7 - 10-4 M; by using horse liver alk. dehydrogenase at 5 x 10-7 - 10-5 M the cofactor regeneration system; by using p-nitroso-N,N'-dimethylamine at 5 x 10-6 - 3 x 10-4 M as th 2nd substrate; and by adding salicyclic acid amide to 2 x 10-4 - 5 x 10-7 M and Na orthophosphate to 0,05 M to the system.deinfo:eu-repo/semantics/openAccessAlkalische Phosphatase570article