Paschke, DietmarEckert, ReinerHülser, Dieter F.2011-12-052016-03-312011-12-052016-03-311992360089836http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-69099http://elib.uni-stuttgart.de/handle/11682/1978http://dx.doi.org/10.18419/opus-1961Antibodies against the main proteins in hepatic gap junctions - connexin-26 and connexin-32 - have been used in conjunction with high-resolution patch-clamp techniques to investigate whether a relation exists between connexin type and conductance of single gap-junctional channels. Two different cell lines, BRL cells, derived from rat liver, and FL cells a human amniotic cell line exhibited the same single-channel conductances in double whole-cell recordings, but reacted differently upon dialysis with antibodies. Preliminary results indicate that both cell lines express mainly connexin-43. Thus, in spite of the inhibitory action of anti-cx26 and anti-cx32 antibodies observed, the data question the reliability of these antibodies for the functional characterization of gap-junction proteins in electrophysiological experiments.eninfo:eu-repo/semantics/openAccessGap junction , Zellkultur , Patch-Clamp-Methode570article