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http://dx.doi.org/10.18419/opus-7765
Autor(en): | Hülser, Dieter F. Webb, Dennis J. |
Titel: | The use of the tip potential of glass microelectrodes in the determination of low cell membrane potentials |
Erscheinungsdatum: | 1973 |
Dokumentart: | Zeitschriftenartikel |
Erschienen in: | Biophysik 10 (1973), Nr. 4, S. 273-280. URL http://dx.doi.org./10.1007/BF01194222 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-67910 http://elib.uni-stuttgart.de/handle/11682/7782 http://dx.doi.org/10.18419/opus-7765 |
Zusammenfassung: | The tip potential of Ling-Gerard glass microelectrodes changes upon insertion into cells and thus impedes the determination of the actual membrane potential. The lower the membrane potential of a cell, the larger will be the error due to this tip potential. However, as is demonstrated, a relationship exists between the tip potential of the electrode and the measured potential difference, which allows the determination of the membrane potential of a particular cell type by linear regression. This method showed that resting lymphocytes had no membrane potential, whereas for the slime mould Dictyostelitium discoideum a membrane potential of about -9 mV could be calculated. |
Enthalten in den Sammlungen: | 15 Fakultätsübergreifend / Sonstige Einrichtung |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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huel17.pdf | 412,67 kB | Adobe PDF | Öffnen/Anzeigen |
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