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http://dx.doi.org/10.18419/opus-7769
Autor(en): | Rajewsky, Manfred F. Fabricius, Erika Hülser, Dieter F. |
Titel: | Synchronisation in vivo: temporary inhibition of DNA synthesis in the rat embryo with hydroxyurea |
Erscheinungsdatum: | 1971 |
Dokumentart: | Zeitschriftenartikel |
Erschienen in: | Experimental cell research 66 (1971), S. 489-492. URL http://dx.doi.org./10.1016/0014-4827(71)90706-3 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-67800 http://elib.uni-stuttgart.de/handle/11682/7786 http://dx.doi.org/10.18419/opus-7769 |
Zusammenfassung: | As part of experimental studies on the synchronisation of mammalian cell populations in vivo, the proliferative response of rat embryo cells (18th day of gestation) was investigated following transplacental administration of hydroxyurea (HU). DNA synthesis was strongly inhibited by i.p. injection of a single dose of 0.25 or 0.5 mg HU/g body weight, for periods of 2.5 and 4.5 h, respectively. Reversal of blocks occurred rapidly due to the short half-life (45 min) of the inhibitor in the embryo. The resulting synchronizing effects were analysed in terms of mitotic indices and rate of 3H-TdR incorporation for a period of 18 h after administration of HU. |
Enthalten in den Sammlungen: | 15 Fakultätsübergreifend / Sonstige Einrichtung |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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huel11.pdf | 314,69 kB | Adobe PDF | Öffnen/Anzeigen |
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