Browsing by Author "Bühle, Peter"

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    Retinoic acid modulates gap junctional permeability: a comparative study of dye spreading and ionic coupling in cultured cells
    (1991) Brümmer, Franz; Zempel, Günther; Bühle, Peter; Stein, Johannes-Christoph; Hülser, Dieter F.
    All-trans retinoic acid (RA), which was recently identified as a morphogen, affects gap junctional permeability in a dose- and time-dependent manner. In five different established mammalian cell lines (FL, BRL, BICR/M1Rk, HEL37, BT5C1) 100 μmol/liter RA reduced Lucifer yellow spreading within 30 min to 20-50% of the control. Ionic coupling, however, remained almost unaffected under the same conditions. Freezefractured membranes of untreated and RA-treated cells were similar with regard to frequency and sizes of gap junction plaques. With concentrations of less than 10 μmol/liter RA the dye spreading increased significantly in the human amniotic cell line FL, pointing to a possible modulatory effect of RA on junctional communication.