Browsing by Author "Busch, Claudia"

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Open Access
Analyse von Stoffwechselprozessen mit der Fermentationskalorimetrie
(1993) Meier-Schneiders, Martin; Grosshans, Ulrich; Busch, Claudia; Eigenberger, Gerhart
Biologische Aktivität ist mit der Freisetzung von Wärmeenergie verbunden. Die Wärmeproduktion ist dabei proportional zur gesamten Stoffwechselaktivität. Da Wärme kontinuierlich entsteht, eignet sie sich zur Nutzung als On-Iine-Signal. Kalorimetrische Methoden werden in den Biowissenschaften bereits seit Jahrzehnten verwendet. Fermentationskalorimetrie ist eine neu entwickelte Meßtechnik, die insbesondere für die kalorimetrische Vermessung biotechnisch relevanter Fermentationen im Labormaßstab gedacht ist.
Open Access
Direct electrodialysis of fermentation broth with periodic removal of fouling layers
(1992) Kuppinger, Franz-Felix; Busch, Claudia; Eigenberger, Gerhart
In the present investigation the fermentation broth is fed directly into the electrodialysis module without prefiltration. Under these conditions a biofouling layer of living and dead parts of the fermentation broth builds up primarily on the anion exchange membranes. The mainly negatively charged fouling producers, for example protein ions, are transported by the electric field to the anion exchange membranes.
Open Access
Investigation and control of fermentation processes using fermenter calorimetry
(1992) Meier-Schneiders, Martin; Grosshans, Ulrich; Busch, Claudia; Eigenberger, Gerhart
It is well known, that heat generation and total metabolic activity in biological systems are strongly correlated. Thus the heat production can provide important information for the study, supervision and control of biological fermentations. Microcalorimeters are widely used for studies concerning physiology, ecology, and pharmacology. They are very sensitive, but the measuring principle exhibits methodical disadvantages for its application in biotechnology (direct control of the fermentation inside of the measuring cell and sampling is impossible). Reaction calorimeters used in chemistry are too insensitive for the detection of small changes in heat generation. Therefore, we developed a new isothermal heat-flux calorimeter-fermenter with a working volume of 2l. The detection level for heat generating processes is 10 mW/l under standard fermenting conditions including agitation, aeration and pH-control. In situ sterilization of the apparatus can be handled easily. The calorimeter is thought to be a versatile tool for laboratory optimization of a fermentation. The aim of the presented work is to discuss what results have been obtained so far and how the heat signal can be used advantangeously in biotechnology.