Browsing by Author "Schmid, Hans-Peter"

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    Perspectives of biogas plants as BECCS facilities : a comparative analysis of biomethane vs. biohydrogen production with carbon capture and storage or use (CCS/CCU)
    (2023) Full, Johannes; Hohmann, Silja; Ziehn, Sonja; Gamero, Edgar; Schließ, Tobias; Schmid, Hans-Peter; Miehe, Robert; Sauer, Alexander
    The transition to a carbon-neutral economy requires innovative solutions that reduce greenhouse gas emissions (GHG) and promote sustainable energy production. Additionally, carbon dioxide removal technologies are urgently needed. The production of biomethane or biohydrogen with carbon dioxide capture and storage are two promising BECCS approaches to achieve these goals. In this study, we compare the advantages and disadvantages of these two approaches regarding their technical, economic, and environmental performance. Our analysis shows that while both approaches have the potential to reduce GHG emissions and increase energy security, the hydrogen-production approach has several advantages, including up to five times higher carbon dioxide removal potential. However, the hydrogen bioenergy with carbon capture and storage (HyBECCS) approach also faces some challenges, such as higher capital costs, the need for additional infrastructure, and lower energy efficiency. Our results give valuable insights into the trade-offs between these two approaches. They can inform decision-makers regarding the most suitable method for reducing GHG emissions and provide renewable energy in different settings.
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    Prosomes (proteasomes) of higher plants
    (1991) Schliephacke, Martin; Kremp, Anette; Schmid, Hans-Peter; Köhler, Kurt; Kull, Ulrich
    From different plant tissues such as tobacco (Nicotiana rustica), potato (Solanum tuberosum), and mung bun (Phaseolus radiatus), ring- or cylinder-shaped particles called prosomes wrere isolated by either sucrose gradient centrifugation or fast protein liquid chromatography (FPLC). These particles have a diameter of 12 to 14 nm and a length of 16 to 18 nm. They migrate under conditions of nondenaturing gel electrophoresis as one distinct band. Sedimentation coefficient and buoyant density in Cs 2SO 4. of the plant prosomes were determined by analytical ultracentrifugation to be approximately 23S and 1.23g/cm 3, respectively. The total molecular mass was estimated by gel filtration to be 650 kDa. Plant prosomes are composed of 12 to 15 proteins with molecular masses in the range of 24 to 35 kDa with isoelectric points of pH 5 to 7 as revealed by twodimensional gel electrophoresis. The protein patterns or prosomes from the three different plant species are very similar. Polyclonal antisera against potato prosomes reacted in Western blots with prosomal proteins of all three plant species. They also bind to some prosomal proteins of animal species. Antisera against animal prosomes react with some proteins of plant prosomes. As shown by lectin blotting, plant prosomes .are glycosylated carrying glucosyl- or mannosyl, and N-acetylgalactosaminyl residues. Prosomal preparations contain non-stoichiometric amounts of small RNA of about 80 kDa. These results suggest that plant prosomes are structurally and functionally homologous to prosomes of other eukaryotic cells.
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    Prosomes exist in plant cells too
    (1986) Kremp, Anette; Schliephacke, Martin; Kull, Ulrich; Schmid, Hans-Peter
    A 19S particle was purified from tobacco (Nicotiana rustica) leaf cells. Its density was determined as 1296 g/cm3 in Cs2SO4-DMSO gradients, indicating the presence of RNA and protein. Polyacrylamide gel electrophoresis (PAGE) revealed eight distinct proteins in the range of 20-30 kD and RNA in the range of 70-80 nucleotides. Electron microscopic examination showed the same raspberry-shaped structure with a central depression as described for prosomes. We conclude that tobacco 19S particles represent small cytoplasmic complexes, possessing biochemical and structural characteristics similar to the hitherto known prosomes of animal cells.
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    Prosomes of higher plants share biochemical properties : [abstract]
    (1988) Schliephacke, Martin; Kremp, Anette; Schmid, Hans-Peter; Kull, Ulrich
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