Browsing by Author "Sempio, Rebecca"

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    Analyzing bioaccessibility of polyphenols in six commercial and six traditional apples (Malus domestica Borkh.) during in vitro and ex vivo oral digestion
    (2023) Kaeswurm, Julia Anna Helene; Sempio, Rebecca; Manca, Federica; Burandt, Melanie Regina; Buchweitz, Maria
    Scope: Apples are an important polyphenol (PP) source. To compare the health benefits of traditional and commercial varieties, the phenolic contents and profiles as well as their release from the matrix (bioaccessibility) during oral digestion are determined. Furthermore, based on these data the proposed beneficial effect of PP on the variety specific allergenicity is discussed. Methods and results: Phenolics are quantified by HPLC‐DAD. Total phenolic contents (TPC) are in the range of 111-645 and 343-1950 mg 100 g−1 dry weight for flesh and peel, respectively. Matrix release during oral digestion is investigated ex vivo, with centrifuged and non‐centrifuged human saliva and in vitro with simulated saliva fluid (SSF). The overall bioaccessibility is similar in all digestion media, ranging between 40-80% and 39-65% of the TPC in flesh and peel, respectively. Analyzing the correlation among Mal‐d 1‐allergen‐content, unoxidized PP, and the allergenic potential for the samples reveals a negligible effect of phenolics. Conclusion: Due to higher phenolic contents in combination with a similar release, increased PP concentrations in the oral phase and an improved uptake of PP from traditional varieties are assumed. However, the proposed beneficial effect of phenolics on allergenicity cannot be confirmed.