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http://dx.doi.org/10.18419/opus-11273
Autor(en): | Kaeswurm, Julia A. H. Könighofer, Lisa Hogg, Melanie Scharinger, Andreas Buchweitz, Maria |
Titel: | Impact of B-ring substitution and acylation with hydroxy cinnamic acids on the inhibition of porcine α-amylase by anthocyanin-3-glycosides |
Erscheinungsdatum: | 2020 |
Dokumentart: | Zeitschriftenartikel |
Seiten: | 21, 6 |
Erschienen in: | Foods 9 (2020), No. 367 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-112908 http://elib.uni-stuttgart.de/handle/11682/11290 http://dx.doi.org/10.18419/opus-11273 |
ISSN: | 2304-8158 |
Zusammenfassung: | An inhibitory effect on alpha-amylase and alpha-glucosidase is postulated for polyphenols. Thus, ingestion of those secondary plant metabolites might reduce postprandial blood glucose level (hyperglycemia), which is a major risk factor for diabetes mellitus type II. In addition to a previous study investigating structure-effect relationships of different phenolic structures, the effect of anthocyanins is studied in detail here, by applying an alpha-amylase activity assay, on the basis of the conversion of 2-chloro-4-nitrophenyl-4-O-ß-galactopyranosyl maltoside (GalG2CNP) and detection of CNP release by UV/Vis spectroscopy and isothermal titration calorimetry (ITC). All anthocyanin-3-glucosides showed a mixed inhibition with a strong competitive proportion, Kic < 134 µM and Kiu < 270 µM; however, the impact of the B-ring substitution was not statistically significant. UV/Vis detection failed to examine the inhibitory effect of acylated cyanidins isolated from black carrot (Daucus carota ssp. Sativus var. Autrorubens Alef.). However, ITC measurements reveal a much stronger inhibitory effect compared to the cyanidin-3-glucoside. Our results support the hypothesis that anthocyanins are effcient alpha-amylase inhibitors and an additional acylation with a cinnamic acid boosts the observed effect. Therefore, an increased consumption of vegetables containing acylated anthocyanin derivatives might help to prevent hyperglycemia. |
Enthalten in den Sammlungen: | 03 Fakultät Chemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Kaeswurm et al. 2020_Impact of B-ring Substitution and acylation with hydroxy cinnamic Acids on inhibition of alpha-amylase by anthocyanins.pdf | Article | 3,78 MB | Adobe PDF | Öffnen/Anzeigen |
Kaeswurm et al. 2020_Foods_supplemental Part.pdf | Supplemental part | 453,78 kB | Adobe PDF | Öffnen/Anzeigen |
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