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http://dx.doi.org/10.18419/opus-14173
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DC Element | Wert | Sprache |
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dc.contributor.author | Tansi, Felista L. | - |
dc.contributor.author | Rüger, Ronny | - |
dc.contributor.author | Böhm, Claudia | - |
dc.contributor.author | Steiniger, Frank | - |
dc.contributor.author | Raasch, Martin | - |
dc.contributor.author | Mosig, Alexander S. | - |
dc.contributor.author | Kontermann, Roland E. | - |
dc.contributor.author | Teichgräber, Ulf K. | - |
dc.contributor.author | Fahr, Alfred | - |
dc.contributor.author | Hilger, Ingrid | - |
dc.date.accessioned | 2024-04-04T14:10:22Z | - |
dc.date.available | 2024-04-04T14:10:22Z | - |
dc.date.issued | 2020 | de |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.other | 1885614179 | - |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-141922 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/14192 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-14173 | - |
dc.description.abstract | Liposomes represent suitable tools for the diagnosis and treatment of a variety of diseases, including cancers. To study the role of the human epidermal growth factor receptor 2 (HER2) as target in cancer imaging and image-guided deliveries, liposomes were encapsulated with an intrinsically quenched concentration of a near-infrared fluorescent dye in their aqueous interior. This resulted in quenched liposomes (termed LipQ), that were fluorescent exclusively upon degradation, dye release, and activation. The liposomes carried an always-on green fluorescent phospholipid in the lipid layer to enable tracking of intact liposomes. Additionally, they were functionalized with single-chain antibody fragments directed to fibroblast activation protein (FAP), a marker of stromal fibroblasts of most epithelial cancers, and to HER2, whose overexpression in 20-30% of all breast cancers and many other cancer types is associated with a poor treatment outcome and relapse. We show that both monospecific (HER2-IL) and bispecific (Bi-FAP/HER2-IL) formulations are quenched and undergo HER2-dependent rapid uptake and cargo release in cultured target cells and tumor models in mice. Thereby, tumor fluorescence was retained in whole-body NIRF imaging for 32-48 h post-injection. Opposed to cell culture studies, Bi-FAP/HER2-IL-based live confocal microscopy of a high HER2-expressing tumor revealed nuclear delivery of the encapsulated dye. Thus, the liposomes have potentials for image-guided nuclear delivery of therapeutics, and also for intraoperative delineation of tumors, metastasis, and tumor margins. | en |
dc.language.iso | en | de |
dc.relation.uri | doi:10.3390/pharmaceutics12100972 | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | de |
dc.subject.ddc | 570 | de |
dc.subject.ddc | 610 | de |
dc.title | Rapid target binding and cargo release of activatable liposomes bearing HER2 and FAP single-chain antibody fragments reveal potentials for image-guided delivery to tumors | en |
dc.type | article | de |
dc.date.updated | 2023-11-14T05:54:19Z | - |
ubs.fakultaet | Energie-, Verfahrens- und Biotechnik | de |
ubs.fakultaet | Fakultätsübergreifend / Sonstige Einrichtung | de |
ubs.institut | Institut für Zellbiologie und Immunologie | de |
ubs.institut | Fakultätsübergreifend / Sonstige Einrichtung | de |
ubs.publikation.seiten | 21 | de |
ubs.publikation.source | Pharmaceutics 12 (2020), No. 0972 | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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pharmaceutics-12-00972.pdf | 3,96 MB | Adobe PDF | Öffnen/Anzeigen |
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