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http://dx.doi.org/10.18419/opus-14679
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DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Guitart Font, Emma | - |
dc.contributor.author | Sprenger, Georg A. | - |
dc.date.accessioned | 2024-07-19T13:37:49Z | - |
dc.date.available | 2024-07-19T13:37:49Z | - |
dc.date.issued | 2020 | de |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-146982 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/14698 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-14679 | - |
dc.description.abstract | Phosphofructokinase (PFK) plays a pivotal role in glycolysis. By deletion of the genes pfkA, pfkB (encoding the two PFK isoenzymes), and zwf (glucose 6-phosphate dehydrogenase) in Escherichia coli K-12, a mutant strain (GL3) with a complete block in glucose catabolism was created. Introduction of plasmid-borne copies of the fsaA wild type gene (encoding E. coli fructose 6-phosphate aldolase, FSAA) did not allow a bypass by splitting fructose 6-phosphate (F6P) into dihydroxyacetone (DHA) and glyceraldehyde 3-phosphate (G3P). Although FSAA enzyme activity was detected, growth on glucose was not reestablished. A mutant allele encoding for FSAA with an amino acid exchange (Ala129Ser) which showed increased catalytic efficiency for F6P, allowed growth on glucose with a µ of about 0.12 h-1. A GL3 derivative with a chromosomally integrated copy of fsaAA129S (GL4) grew with 0.05 h-1 on glucose. A mutant strain from GL4 where dhaKLM genes were deleted (GL5) excreted DHA. By deletion of the gene glpK (glycerol kinase) and overexpression of gldA (of glycerol dehydrogenase), a strain (GL7) was created which showed glycerol formation (21.8 mM; yield approximately 70% of the theoretically maximal value) as main end product when grown on glucose. A new-to-nature pathway from glucose to glycerol was created. | en |
dc.description.sponsorship | Ministerium für Wissenschaft und Kunst of Baden-Württemberg | de |
dc.language.iso | en | de |
dc.relation.uri | doi:10.3390/ijms21249625 | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | de |
dc.subject.ddc | 570 | de |
dc.title | Opening a novel biosynthetic pathway to dihydroxyacetone and glycerol in Escherichia coli mutants through expression of a gene variant (fsaAA129S) for fructose 6-phosphate aldolase | en |
dc.type | article | de |
dc.date.updated | 2023-11-14T01:28:17Z | - |
ubs.fakultaet | Energie-, Verfahrens- und Biotechnik | de |
ubs.institut | Institut für Mikrobiologie | de |
ubs.publikation.noppn | yes | de |
ubs.publikation.seiten | 22 | de |
ubs.publikation.source | International journal of molecular sciences 21 (2020), No. 9625 | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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ijms-21-09625.pdf | 2,97 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons