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Authors: Brenner, Joachim
Zempel, Günther
Hülser, Dieter F.
Title: Production of tissue plasminogen activator (t-PA) with differentiated F9-embryonic carcinoma cells grown as multicell spheroids
Issue Date: 1991 Konferenzbeitrag Reuß, Matthias (Hrsg.): Biochemical engineering - Stuttgart : proceedings of the 2. International Symposium on Biochemical Engineering which was held at the Univ. of Stuttgart from March 5 to 7, 1990. Stuttgart : G. Fischer, 1991. - ISBN 3-437-30662-6, S. 220-223
Abstract: F9 cells can only temporarily be cultivated as single cell suspension. The growth of multicell spheroids from a single cell suspension is shown. Within 24 hours the cells aggregated to multicell spheroids in spinner flasks. The aggregation was almost finished within 24 h and was independent of the initial cell concentration. but could be influenced by the geometry of the cultu re flask and the stirring velocity. A significant cell proliferation of the anchorage-dependent F9 cells was only detectable in the aggregated state. A narrow size distribution of multicell spheroids in a 3 days old culture revealed identical spheroid sizes. Under our conditions. with an inital cell density of 10 5 cells/ml a cell concentration of 6x10 6 cells/ml was reached within 4 days of spheroid culture. Cells in monolayers and in multicell spheroids were well coupled by gap junctions. Differentiated F9 cells showed a fibroblastoid morphology in contrast to the epitheloid morphology of undifferentiated F9 stem cells. This was not the case for multicell spheroids where the cells were also well coupled.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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