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Authors: Bräuner, Thomas
Hülser, Dieter F.
Title: Tumor cell invasion and gap junctional communication. 2, Normal and malignant cells confronted in multicell spheroids
Issue Date: 1990 Zeitschriftenartikel Invasion metastasis 10 (1990), S. 31-48
Abstract: The invasive behavior of five tumor cell lines was investigated with an in vitro invasion assay developed by Mareel et al. Spheroids of all 5 cell lines readily attached to precultured heart fragments (PHF), resulting in confronting pairs. Tumor cells (8ICR/MIR k C6, and EMT6/Ro) which communicated with the host tissue via gap junctions, rapidly invaded the PHF within 3 days. Population doubling time or migratory activities had no influence on the invasion process. The noncoupled HeLa and L cells formed a cellular capsule around the PHF and showed no invasive activities. HeLa cells, however, started to destroy the PHF after 4 days. We suggest a mechanism different from that of coupled tumor cells. Epithelioid HeLa cells are linked by numerous tight junctions and may, therefore, cut off the nutrition supply for the inner-laying PHF, resulting in a disintegration of the heart tissue.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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