Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
http://dx.doi.org/10.18419/opus-1958
Langanzeige der Metadaten
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Eckert, Reiner | de |
dc.contributor.author | Dunina-Barkovskaja, Antonina | de |
dc.contributor.author | Hülser, Dieter F. | de |
dc.date.accessioned | 2011-12-05 | de |
dc.date.accessioned | 2016-03-31T07:53:04Z | - |
dc.date.available | 2011-12-05 | de |
dc.date.available | 2016-03-31T07:53:04Z | - |
dc.date.issued | 1993 | de |
dc.identifier.other | 363923489 | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-69134 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/1975 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-1958 | - |
dc.description.abstract | HeLa cells seem not to be junctionally coupled when probed with techniques such as Lucifer yellow spreading and/or ionic coupling measured with three inserted microelectrodes. When investigated with double whole-cell patch-clamp measurements, HeLa cells in monolayer cultures were electrically coupled in 39% of the cases with very low transjunctional conductances (average one to five open channels). These gapjunction channels had a single-channel conductance γ=26±6 pS and were voltage-gated with an equivalent gating charge ζ=3.1±1.5 for a voltage of half-maximal inactivation Uo=49±10 mV. The voltage-dependent component represents only 31±8% of the total junctional conductance. The voltage-insensitive conductance is characterized by a residual open probability po(∞)=0.34±0.12, which corresponds to a ratio Gmin/Gmax=0.50±0.12. Dissociation of monolayer cells into cell pairs yielded about 58% coupled cell pairs with no notably altered single-channel properties. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Zellkommunikation , Gap junction , HeLa-Zelle | de |
dc.subject.ddc | 570 | de |
dc.title | Biophysical characterization of gap-junction channels in HeLa cells | en |
dc.type | article | de |
dc.date.updated | 2011-12-05 | de |
ubs.fakultaet | Fakultät Energie-, Verfahrens- und Biotechnik | de |
ubs.fakultaet | Fakultätsübergreifend / Sonstige Einrichtung | de |
ubs.institut | Institut für Biomaterialien und biomolekulare Systeme | de |
ubs.institut | Sonstige Einrichtung | de |
ubs.opusid | 6913 | de |
ubs.publikation.source | Pflügers Archiv 424 (1993), S. 335-342. URL http://dx.doi.org./10.1007/BF00384361 | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
huel83.pdf | 1,09 MB | Adobe PDF | Öffnen/Anzeigen |
Alle Ressourcen in diesem Repositorium sind urheberrechtlich geschützt.