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http://dx.doi.org/10.18419/opus-2012
Autor(en): | Rubio, Miguel Angel Engesser, Karl-Heinrich Knackmuss, Hans-Joachim |
Titel: | Microbial metabolism of chlorosalicylates: accelerated evolution by natural genetic exchange |
Erscheinungsdatum: | 1986 |
Dokumentart: | Zeitschriftenartikel |
Erschienen in: | Archives of microbiology 145 (1986), S. 116-122. URL http://dx.doi.org./10.1007/BF00446767 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76572 http://elib.uni-stuttgart.de/handle/11682/2029 http://dx.doi.org/10.18419/opus-2012 |
Zusammenfassung: | Methylsalicylate-grown cells of Pseudomonas sp. WR 401 cometabolized 3-, 4- and 5-substituted halosalicylates to the corresponding halocatechols. Further degradation was unproductive due to the presence of high levels of catechol 2,3-dioxygenase. This strain acquired the ability to utilize 3-chlorobenzoate following acquisition of genes from Pseudomonas sp. B 13 which are necessary for the assimilation of chlorocatechols. This derivative (WR 4011) was unable to use 4- or 5-chlorosalicylates. Derivatives able to use these compounds were obtained by plating WR 4011 on 5-chlorosalicylate minimal medium; one such derivative was designated WR 4016. The acquisition of this property was accompanied by concomitant loss of the methylsalicylate phenotype. During growth on 4- or 5-chlorosalicylate the typical enzymes of chlorocatechol assimilation were detected in cell free extracts, whereas catechol 2,3-dioxygenase activity was not induced. Repeated subcultivation of WR 4016 in the presence of 3-chlorosalicylate produced variants (WR 4016-1) which grew on all three isomers. |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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eng34.pdf | 2,2 MB | Adobe PDF | Öffnen/Anzeigen |
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