Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-816
Authors: Momoi, Kyoko
Hofmann, Ute
Schmid, Rolf D.
Urlacher, Vlada B.
Title: Reconstitution of beta-carotene hydroxylase activity of thermostable CYP175A1 monooxygenase
Issue Date: 2006
metadata.ubs.publikation.typ: Preprint
metadata.ubs.publikation.source: Biochemical and biophysical research communications 339 (2006), S. 331-336. URL http://dx.doi.org/10.1016/j.bbrc.2005.11.023
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26567
http://elib.uni-stuttgart.de/handle/11682/833
http://dx.doi.org/10.18419/opus-816
Abstract: CYP175A1 is a thermostable P450 Monooxygenase from Thermus thermophilus HB27, demonstrating in vivo activity towards -carotene. Activity of CYP175A1 was reconstituted in vitro using artificial electron transport proteins. First results were obtained in the mixture with a crude E. coli cell extract at 37°C. In this system -carotene was hydroxylated to -cryptoxanthin. The result indicated the presence of electron transport enzymes among the E. coli proteins, which are suitable for CYP175A1. However, upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from E. coli, only very low -cryptoxanthin production was observed. Remarkably, with another artificial electron transport system, putidaredoxin and putidaredoxin reductase from Pseudomonas putida, purified CYP175A1 enzyme hydroxylated -carotene at 3- and also 3’-positions, resulting in -cryptoxanthin and zeaxanthin. Under the optimal reaction conditions, the turnover rate of the enzyme reached 0.23 nmol -cryptoxanthin produced per nmol P450 per min.
Appears in Collections:03 Fakultät Chemie

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