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http://dx.doi.org/10.18419/opus-841
Autor(en): | Roh, Changhyun Villatte, Francois Kim, Byung-Gee Schmid, Rolf D. |
Titel: | "In gel patch electrophoresis" : a new method for environmental DNA purification |
Erscheinungsdatum: | 2005 |
Dokumentart: | Preprint |
Erschienen in: | Electrophoresis 26 (2005), S. 3055-3061. URL http://dx.doi.org/10.1002/elps.200410328 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26909 http://elib.uni-stuttgart.de/handle/11682/858 http://dx.doi.org/10.18419/opus-841 |
Zusammenfassung: | Most of the microorganism species are largely untapped and could represent an interesting reservoir of genes useful for biotechnological applications. Unfortunately, a major difficulty associated with the methods used to isolate environmental DNA is related to the contamination of the extracted material with humic substances. These polyphenolic compounds inhibit the DNA processing reactions and severely impede cloning procedures. In this work, we describe a rapid, simple and efficient method for the purification of genomic DNA from environmental samples: we added a chromatography step directly embedded into an agarose gel electrophoresis. This strategy enabled the DNA extraction from various environmental samples and it appeared that the purity grade was compatible with digestion by restriction enzymes and PCR amplifications. |
Enthalten in den Sammlungen: | 03 Fakultät Chemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Roh_2005.pdf | 425,1 kB | Adobe PDF | Öffnen/Anzeigen |
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