Enantioselective hydrolysis of O-acetylmandelonitrile to O-acetylmandelic acid by bacterial nitrilases

Layh, Norman; Stolz, Andreas; Förster, Siegfried; Effenberger, Franz; Knackmuss, Hans-Joachim

Enantioselective hydrolysis of O-acetylmandelonitrile to O-acetylmandelic acid by bacterial nitrilases

dc.contributor.author	Layh, Norman	de
dc.contributor.author	Stolz, Andreas	de
dc.contributor.author	Förster, Siegfried	de
dc.contributor.author	Effenberger, Franz	de
dc.contributor.author	Knackmuss, Hans-Joachim	de
dc.date.accessioned	2009-08-28	de
dc.date.accessioned	2016-03-31T07:52:29Z
dc.date.available	2009-08-28	de
dc.date.available	2016-03-31T07:52:29Z
dc.date.issued	1992	de
dc.date.updated	2010-09-30	de
dc.description.abstract	Bacteria were enriched from soil samples, using benzylcyanide, α-methyl-, α-ethyl- or α-methoxybenzyl-cyanide as the sole source of nitrogen. All isolated strains belonged to the genus Pseudomonas. Resting cells of the isolates hydrolysed O-acetylmandelonitrile to O-acetylmandelic acid, O-acetylmandelic acid amide and mandelic acid. From racemic O-acetylmandelonitrile all isolates preferentially formed R(–)-acetylmandelic acid ( = d-acetylmandelic acid). The enantioselective hydrolysis of O-acetylmandelonitrile could also be demonstrated in vitro. Crude extracts did not hydrolyse O-acetylmandelic acid amide indicating an enantioselective nitrilase rather than a nitrile hydratase/amidase system.	en
dc.identifier.other	316208698	de
dc.identifier.uri	http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-44371	de
dc.identifier.uri	http://elib.uni-stuttgart.de/handle/11682/1831
dc.identifier.uri	http://dx.doi.org/10.18419/opus-1814
dc.language.iso	en	de
dc.rights	info:eu-repo/semantics/openAccess	de
dc.subject.classification	Nitrile , Pseudomonas , Enantioselektivität , Mandelsäurederivate	de
dc.subject.ddc	570	de
dc.title	Enantioselective hydrolysis of O-acetylmandelonitrile to O-acetylmandelic acid by bacterial nitrilases	en
dc.type	article	de
ubs.fakultaet	Fakultät Energie-, Verfahrens- und Biotechnik	de
ubs.fakultaet	Fakultät Chemie	de
ubs.institut	Institut für Mikrobiologie	de
ubs.institut	Institut für Organische Chemie	de
ubs.opusid	4437	de
ubs.publikation.source	Archives of Microbiology 158 (1992), S. 405-411. URL http://dx.doi.org./10.1007/BF00276300	de
ubs.publikation.typ	Zeitschriftenartikel	de

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