Detection of plant virus particles with a capacitive field-effect sensor

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dc.contributor.authorJablonski, Melanie
dc.contributor.authorPoghossian, Arshak
dc.contributor.authorKeusgen, Michael
dc.contributor.authorWege, Christina
dc.contributor.authorSchöning, Michael J.
dc.date.accessioned2023-04-14T12:32:50Z
dc.date.available2023-04-14T12:32:50Z
dc.date.issued2021de
dc.date.updated2023-03-24T17:40:08Z
dc.description.abstractPlant viruses are major contributors to crop losses and induce high economic costs worldwide. For reliable, on-site and early detection of plant viral diseases, portable biosensors are of great interest. In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip surface was observed. Additionally, the isoelectric point of the biotinylated TMV particles was determined via zeta potential measurements and the influence of ionic strength of the measurement solution on the TMV-modified EIS sensor signal has been studied.en
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG, German Research Foundation)de
dc.description.sponsorshipProjekt DEALde
dc.identifier.issn1618-2642
dc.identifier.issn1618-2650
dc.identifier.other184349325X
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-129631de
dc.identifier.urihttp://elib.uni-stuttgart.de/handle/11682/12963
dc.identifier.urihttp://dx.doi.org/10.18419/opus-12944
dc.language.isoende
dc.relation.uridoi:10.1007/s00216-021-03448-8de
dc.rightsinfo:eu-repo/semantics/openAccessde
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de
dc.subject.ddc580de
dc.titleDetection of plant virus particles with a capacitive field-effect sensoren
dc.typearticlede
ubs.fakultaetEnergie-, Verfahrens- und Biotechnikde
ubs.fakultaetFakultätsübergreifend / Sonstige Einrichtungde
ubs.institutInstitut für Biomaterialien und biomolekulare Systemede
ubs.institutFakultätsübergreifend / Sonstige Einrichtungde
ubs.publikation.seiten5669-5678de
ubs.publikation.sourceAnalytical and bioanalytical chemistry 413 (2021), S. 5669-5678de
ubs.publikation.typZeitschriftenartikelde

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