"In gel patch electrophoresis" : a new method for environmental DNA purification

Roh, Changhyun; Villatte, Francois; Kim, Byung-Gee; Schmid, Rolf D.

"In gel patch electrophoresis" : a new method for environmental DNA purification

dc.contributor.author	Roh, Changhyun	de
dc.contributor.author	Villatte, Francois	de
dc.contributor.author	Kim, Byung-Gee	de
dc.contributor.author	Schmid, Rolf D.	de
dc.date.accessioned	2006-06-13	de
dc.date.accessioned	2016-03-31T07:46:46Z
dc.date.available	2006-06-13	de
dc.date.available	2016-03-31T07:46:46Z
dc.date.issued	2005	de
dc.date.updated	2015-12-11	de
dc.description.abstract	Most of the microorganism species are largely untapped and could represent an interesting reservoir of genes useful for biotechnological applications. Unfortunately, a major difficulty associated with the methods used to isolate environmental DNA is related to the contamination of the extracted material with humic substances. These polyphenolic compounds inhibit the DNA processing reactions and severely impede cloning procedures. In this work, we describe a rapid, simple and efficient method for the purification of genomic DNA from environmental samples: we added a chromatography step directly embedded into an agarose gel electrophoresis. This strategy enabled the DNA extraction from various environmental samples and it appeared that the purity grade was compatible with digestion by restriction enzymes and PCR amplifications.	en
dc.identifier.other	262583852	de
dc.identifier.uri	http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26909	de
dc.identifier.uri	http://elib.uni-stuttgart.de/handle/11682/858
dc.identifier.uri	http://dx.doi.org/10.18419/opus-841
dc.language.iso	en	de
dc.rights	info:eu-repo/semantics/openAccess	de
dc.subject.classification	Elektrophorese , Agargel-Elektrophorese	de
dc.subject.ddc	540	de
dc.subject.other	in gel patch electrophoresis , purification , metagenome	en
dc.title	"In gel patch electrophoresis" : a new method for environmental DNA purification	en
dc.type	preprint	de
ubs.fakultaet	Fakultät Chemie	de
ubs.fakultaet	Fakultätsübergreifend / Sonstige Einrichtung	de
ubs.institut	Institut für Technische Biochemie	de
ubs.institut	Sonstige Einrichtung	de
ubs.opusid	2690	de
ubs.publikation.source	Electrophoresis 26 (2005), S. 3055-3061. URL http://dx.doi.org/10.1002/elps.200410328	de
ubs.publikation.typ	Preprint	de

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