Comparing three different Phaeodactylum tricornutum strains for the production of chrysolaminarin in flat panel airlift photobioreactors

Abstract

In recent years, various applications for algae-based ß-1,3-glucans have been postulated, including animal feed and human nutrition. Chrysolaminarin is a ß-1,3-1,6-glucan produced by diatoms such as Phaeodactylum tricornutum for energy storage. It is accumulated under nutrient-depleted cultivation conditions. In this study, the production of chrysolaminarin in artificially illuminated scalable flat panel airlift photobioreactors (FPA) was investigated by using P. tricornutum in a two-stage production process. In the growth stage primarily biomass is produced, and the subsequent nitrogen-depleted stage induces the accumulation of chrysolaminarin. Three P. tricornutum strains (SAG 1090-1a, SAG 1090-1b, SAG 1090-6) were cultured at laboratory scale in 6 L-FPA reactors under controlled light conditions to characterize the process and identify a production strain. The chrysolaminarin content of the algae strains was analysed and additionally their contents of eicosapentaenoic acid and fucoxanthin, both of which could be involved in a possible co-production. Strain SAG 1090-1b exhibited the highest biomass productivity and chrysolaminarin content (317 ± 9 mg gDW-1) after nitrogen depletion, and thus stood out as the most suitable for chrysolaminarin production in a two-stage process. A co-production of the three compounds is possible. However, during nitrogen depletion there occurred trade-offs between the compounds. As chrysolaminarin was produced, the amount of fucoxanthin in the culture stagnated or even decreased depending on the selected strain.