Universität Stuttgart
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Item Open Access Editorial - optical microscopic and spectroscopic techniques targeting biological applications(2021) Micó, Vicente; Pedrini, Giancarlo; Lei, Ming; Zuo, Chao; Gao, PengItem Open Access Double pulse-electronic speckle interferometry(1993) Pedrini, Giancarlo; Pfister, Berthold P.; Tiziani, Hans J.In this paper we describe a double-pulsed electronic speckle interferometry system. Two separated speckle patterns are recorded within few microseconds using a charge-coupled device camera, the two images are then stored in a frame grabber and the subtraction method is applied. A quantitative analysis of the fringes using the spatial-carrier phase-shift method is presented. The results show that this method is well suited to study transient vibrations.Item Open Access Opposed-view dark-field digital holographic microscopy(2014) Faridian, Ahmad; Pedrini, Giancarlo; Osten, WolfgangScattering and absorption belong to the major problems in imaging the internal layers of a biological specimen. Due to the structural inhomogeneity of the specimen, the distribution of the structures in the upper layers of a given internal structure of interest is different from the lower layers that may result in different interception of scattered light, falling into the angular aperture of the microscope objective, from the object in each imaging view. Therefore, different spatial frequencies of the scattered light can be acquired from different (top and bottom) views. We have arranged an opposed-view dark-field digital holographic microscope (DHM) to collect the scattered light concurrently from both views with the aim to increase the contrast of internal structures and improve the signal-to-noise ratio. Implementing a DHM system gives the possibility to implement digital refocusing process and obtain multilayer images from each side without a depth scan of the object. The method is explained and the results are presented exemplary for a Drosophila embryo.Item Open Access Advantages of holographic imaging through fog(2023) Gröger, Alexander; Pedrini, Giancarlo; Claus, Daniel; Alekseenko, Igor; Gloeckler, Felix; Reichelt, StephanItem Open Access Two-wavelength digital holography through fog(2023) Gröger, Alexander; Pedrini, Giancarlo; Fischer, Felix; Claus, Daniel; Aleksenko, Igor; Reichelt, Stephan