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Autor(en): Lange, Julian
Münch, Eugenia
Müller, Jan
Busche, Tobias
Kalinowski, Jörn
Takors, Ralf
Blombach, Bastian
Titel: Deciphering the adaptation of Corynebacterium glutamicum in transition from aerobiosis via microaerobiosis to anaerobiosis
Erscheinungsdatum: 2018
Dokumentart: Zeitschriftenartikel
Seiten: 32
Erschienen in: Genes 9 (2018), No. 297
URI: http://elib.uni-stuttgart.de/handle/11682/10028
http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-100289
http://dx.doi.org/10.18419/opus-10011
ISSN: 2073-4425
Zusammenfassung: Zero-growth processes are a promising strategy for the production of reduced molecules and depict a steady transition from aerobic to anaerobic conditions. To investigate the adaptation of Corynebacterium glutamicum to altering oxygen availabilities, we conceived a triple-phase fermentation process that describes a gradual reduction of dissolved oxygen with a shift from aerobiosis via microaerobiosis to anaerobiosis. The distinct process phases were clearly bordered by the bacteria’s physiologic response such as reduced growth rate, biomass substrate yield and altered yield of fermentation products. During the process, sequential samples were drawn at six points and analyzed via RNA-sequencing, for metabolite concentrations and for enzyme activities. We found transcriptional alterations of almost 50% (1421 genes) of the entire protein coding genes and observed an upregulation of fermentative pathways, a rearrangement of respiration, and mitigation of the basic cellular mechanisms such as transcription, translation and replication as a transient response related to the installed oxygen dependent process phases. To investigate the regulatory regime, 18 transcriptionally altered (putative) transcriptional regulators were deleted, but none of the deletion strains showed noticeable growth kinetics under an oxygen restricted environment. However, the described transcriptional adaptation of C. glutamicum resolved to varying oxygen availabilities provides a useful basis for future process and strain engineering.
Enthalten in den Sammlungen:04 Fakultät Energie-, Verfahrens- und Biotechnik

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