Please use this identifier to cite or link to this item:
http://dx.doi.org/10.18419/opus-13503
Authors: | Hermann, Maria Teleki, Attila Weitz, Sandra Niess, Alexander Freund, Andreas Bengelsdorf, Frank Robert Dürre, Peter Takors, Ralf |
Title: | Identifying and engineering bottlenecks of autotrophic isobutanol formation in recombinant C. ljungdahlii by systemic analysis |
Issue Date: | 2021 |
metadata.ubs.publikation.typ: | Zeitschriftenartikel |
metadata.ubs.publikation.seiten: | 14 |
metadata.ubs.publikation.source: | Frontiers in bioengineering and biotechnology 9 (2021), No. 647853 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-135228 http://elib.uni-stuttgart.de/handle/11682/13522 http://dx.doi.org/10.18419/opus-13503 |
ISSN: | 2296-4185 |
Abstract: | Clostridium ljungdahlii (C. ljungdahlii, CLJU) is natively endowed producing acetic acid, 2,3-butandiol, and ethanol consuming gas mixtures of CO2, CO, and H2 (syngas). Here, we present the syngas-based isobutanol formation using C. ljungdahlii harboring the recombinant amplification of the “Ehrlich” pathway that converts intracellular KIV to isobutanol. Autotrophic isobutanol production was studied analyzing two different strains in 3-L gassed and stirred bioreactors. Physiological characterization was thoroughly applied together with metabolic profiling and flux balance analysis. Thereof, KIV and pyruvate supply were identified as key “bottlenecking” precursors limiting preliminary isobutanol formation in CLJU[KAIA] to 0.02 g L-1. Additional blocking of valine synthesis in CLJU[KAIA]:ilvE increased isobutanol production by factor 6.5 finally reaching 0.13 g L-1. Future metabolic engineering should focus on debottlenecking NADPH availability, whereas NADH supply is already equilibrated in the current generation of strains. |
Appears in Collections: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Files in This Item:
File | Description | Size | Format | |
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Data_Sheet_1.pdf | Supplement | 234,42 kB | Adobe PDF | View/Open |
fbioe-09-647853.pdf | Artikel | 3,85 MB | Adobe PDF | View/Open |
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