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http://dx.doi.org/10.18419/opus-14638
Autor(en): | Verhagen, Natascha Wijaya, Andy Wiranata Teleki, Attila Fadhlullah, Muhammad Unsöld, Andreas Schilling, Martin Heinrich, Christoph Takors, Ralf |
Titel: | Comparison of l‐tyrosine containing dipeptides reveals maximum ATP availability for l‐prolyl‐l‐tyrosine in CHO cells |
Erscheinungsdatum: | 2020 |
Dokumentart: | Zeitschriftenartikel |
Seiten: | 384-394 |
Erschienen in: | Engineering in life sciences 20 (2020), S. 384-394 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-146572 http://elib.uni-stuttgart.de/handle/11682/14657 http://dx.doi.org/10.18419/opus-14638 |
ISSN: | 1618-2863 1618-0240 |
Zusammenfassung: | Increasing markets for biopharmaceuticals, including monoclonal antibodies, have triggered a permanent need for bioprocess optimization. Biochemical engineering approaches often include the optimization of basal and feed media to improve productivities of Chinese hamster ovary (CHO) cell cultures. Often, l‐tyrosine is added as dipeptide to deal with its poor solubility at neutral pH. Showcasing IgG1 production with CHO cells, we investigated the supplementation of three l‐tyrosine (TYR, Y) containing dipeptides: glycyl‐l‐tyrosine (GY), l‐tyrosyl‐l‐valine (YV), and l‐prolyl‐l‐tyrosine (PY). While GY and YV led to almost no phenotypic and metabolic differences compared to reference samples, PY significantly amplified TYR uptake thus maximizing related catabolic activity. Consequently, ATP formation was roughly four times higher upon PY application than in reference samples. |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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ELSC_ELSC1323.pdf | 2,51 MB | Adobe PDF | Öffnen/Anzeigen |
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