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http://dx.doi.org/10.18419/opus-14748
Autor(en): | Harting, Claudia Teleki, Attila Braakmann, Marius Jankowitsch, Frank Takors, Ralf |
Titel: | Systemic intracellular analysis for balancing complex biosynthesis in a transcriptionally deregulated Escherichia coli l‐Methionine producer |
Erscheinungsdatum: | 2024 |
Dokumentart: | Zeitschriftenartikel |
Seiten: | 15 |
Erschienen in: | Microbial biotechnology 17 (2024), No. e14433 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-147675 http://elib.uni-stuttgart.de/handle/11682/14767 http://dx.doi.org/10.18419/opus-14748 |
ISSN: | 1751-7915 1751-7915 |
Zusammenfassung: | l-Methionine (l-Met) has gained remarkable interest due to its multifaceted and versatile applications in the fields of nutrition, pharmaceuticals and clinical practice. In this study, the fluxes of the challenging l-Met biosynthesis in the producer strain Escherichia coli (E. coli) DM2853 were fine-tuned to enable improved l-Met production. The potential bottlenecks identified in sulfur assimilation and l-Met synthesis downstream of O-succinyl-l-homoserine (OSHS) were addressed by overexpressing glutaredoxin 1 (grxA), thiosulfate sulfurtransferase (pspE) and O-succinylhomoserine lyase (metB). Although deemed as a straightforward target for improving glucose-to-Met conversion, the yields remained at approximately 12%-13% (g/g). Instead, intracellular l-Met pools increased by up to four-fold with accelerated kinetics. Overexpression of the Met exporter ygaZH may serve as a proper valve for releasing the rising internal Met pressure. Interestingly, the export kinetics revealed maximum saturated export rates already at low growth rates. This scenario is particularly advantageous for large-scale fermentation when product formation is ideally uncoupled from biomass formation to achieve maximum performance within the technical limits of large-scale bioreactors. |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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MBT2_MBT214433.pdf | 7,37 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons