Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-14780
Authors: Wendlandt, Tim
Britz, Beate
Kleinow, Tatjana
Hipp, Katharina
Eber, Fabian J.
Wege, Christina
Title: Getting hold of the tobamovirus particle : why and how? Purification routes over time and a new customizable approach
Issue Date: 2024
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.seiten: 42
metadata.ubs.publikation.source: Viruses 16 (2024), No. 884
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-147996
http://elib.uni-stuttgart.de/handle/11682/14799
http://dx.doi.org/10.18419/opus-14780
ISSN: 1999-4915
Abstract: This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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