Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-1814
Authors: Layh, Norman
Stolz, Andreas
Förster, Siegfried
Effenberger, Franz
Knackmuss, Hans-Joachim
Title: Enantioselective hydrolysis of O-acetylmandelonitrile to O-acetylmandelic acid by bacterial nitrilases
Issue Date: 1992
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.source: Archives of Microbiology 158 (1992), S. 405-411. URL http://dx.doi.org./10.1007/BF00276300
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-44371
http://elib.uni-stuttgart.de/handle/11682/1831
http://dx.doi.org/10.18419/opus-1814
Abstract: Bacteria were enriched from soil samples, using benzylcyanide, α-methyl-, α-ethyl- or α-methoxybenzyl-cyanide as the sole source of nitrogen. All isolated strains belonged to the genus Pseudomonas. Resting cells of the isolates hydrolysed O-acetylmandelonitrile to O-acetylmandelic acid, O-acetylmandelic acid amide and mandelic acid. From racemic O-acetylmandelonitrile all isolates preferentially formed R(–)-acetylmandelic acid ( = d-acetylmandelic acid). The enantioselective hydrolysis of O-acetylmandelonitrile could also be demonstrated in vitro. Crude extracts did not hydrolyse O-acetylmandelic acid amide indicating an enantioselective nitrilase rather than a nitrile hydratase/amidase system.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

Files in This Item:
File Description SizeFormat 
eff231.pdf603,51 kBAdobe PDFView/Open


Items in OPUS are protected by copyright, with all rights reserved, unless otherwise indicated.