Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-1921
Authors: Krähling, Hermann
Schinkewitz, Ursula
Barker, Angela
Hülser, Dieter F.
Title: Electronmicroscopical and electrophysiological investigations on polyethylene glycol induced cell fusion
Issue Date: 1978
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.source: Cytobiologie 17 (1978), S. 51-61
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68139
http://elib.uni-stuttgart.de/handle/11682/1938
http://dx.doi.org/10.18419/opus-1921
Abstract: Cells of monolayer cultures are fused by high concentrations of polyethylene glycol (PEG) with a molecular weight of approximately 1500. This process is independent of extracellular ca++ions. PEG changes transiently the surface membrane and leads to fusion only after replacing it by normal medium. Before the final fusion of two cells, the onset of ionic coupling via longer lasting pseudopodial contact can be measured. Only cells that are synchronous in the secretory and pseudopodial response to PEG may fuse with each other.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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