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dc.contributor.authorBrenner, Joachimde
dc.contributor.authorZempel, Güntherde
dc.contributor.authorHülser, Dieter
dc.description.abstractF9 cells can only temporarily be cultivated as single cell suspension. The growth of multicell spheroids from a single cell suspension is shown. Within 24 hours the cells aggregated to multicell spheroids in spinner flasks. The aggregation was almost finished within 24 h and was independent of the initial cell concentration. but could be influenced by the geometry of the cultu re flask and the stirring velocity. A significant cell proliferation of the anchorage-dependent F9 cells was only detectable in the aggregated state. A narrow size distribution of multicell spheroids in a 3 days old culture revealed identical spheroid sizes. Under our conditions. with an inital cell density of 10 5 cells/ml a cell concentration of 6x10 6 cells/ml was reached within 4 days of spheroid culture. Cells in monolayers and in multicell spheroids were well coupled by gap junctions. Differentiated F9 cells showed a fibroblastoid morphology in contrast to the epitheloid morphology of undifferentiated F9 stem cells. This was not the case for multicell spheroids where the cells were also well coupled.en
dc.subject.classificationZellkultur , Gap junction , Gewebsplasminogen-Aktivatorde
dc.titleProduction of tissue plasminogen activator (t-PA) with differentiated F9-embryonic carcinoma cells grown as multicell spheroidsen
ubs.fakultaetFakultät Energie-, Verfahrens- und Biotechnikde
ubs.institutInstitut für Biomaterialien und biomolekulare Systemede
ubs.publikation.sourceReuß, Matthias (Hrsg.): Biochemical engineering - Stuttgart : proceedings of the 2. International Symposium on Biochemical Engineering which was held at the Univ. of Stuttgart from March 5 to 7, 1990. Stuttgart : G. Fischer, 1991. - ISBN 3-437-30662-6, S. 220-223de
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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