Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-1951
Authors: Brenner, Joachim
Hülser, Dieter F.
Title: A rapid method for the determination of absolute particle sizes in a flow cytometer
Issue Date: 1991
metadata.ubs.publikation.typ: Buchbeitrag
metadata.ubs.publikation.source: Schütt, Wolfgang (Hrsg.): Physical characterization of biological cells. Berlin : Verl. Gesundheit, 1991. - ISBN 3-333-00632-4, S. 131-146
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68913
http://elib.uni-stuttgart.de/handle/11682/1968
http://dx.doi.org/10.18419/opus-1951
Abstract: The most precise determination of a cell-size distribution can be expected by registering the signal of a three-dimensional size (particle volume). This is best performed in a flow cytometer with an integrated volume measurement according to the Coulter-principle.This electrical measurement is very fast and is more precise than the forward-scatter method which is used alternativly in flow cytometry. The mean size of particles which has been determined by the Coulter-principle is mostly given by arbitrary units. We, therefore, present here a simple and fast calibration method which allows the transformation of arbitrary to absolute values. In addition we compared these data with one- and two-dimensional measurements under the microscope and an image-processing-system, respectively.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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