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http://dx.doi.org/10.18419/opus-1951
| Autor(en): | Brenner, Joachim Hülser, Dieter F. |
| Titel: | A rapid method for the determination of absolute particle sizes in a flow cytometer |
| Erscheinungsdatum: | 1991 |
| Dokumentart: | Buchbeitrag |
| Erschienen in: | Schütt, Wolfgang (Hrsg.): Physical characterization of biological cells. Berlin : Verl. Gesundheit, 1991. - ISBN 3-333-00632-4, S. 131-146 |
| URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68913 http://elib.uni-stuttgart.de/handle/11682/1968 http://dx.doi.org/10.18419/opus-1951 |
| Zusammenfassung: | The most precise determination of a cell-size distribution can be expected by registering the signal of a three-dimensional size (particle volume). This is best performed in a flow cytometer with an integrated volume measurement according to the Coulter-principle.This electrical measurement is very fast and is more precise than the forward-scatter method which is used alternativly in flow cytometry. The mean size of particles which has been determined by the Coulter-principle is mostly given by arbitrary units. We, therefore, present here a simple and fast calibration method which allows the transformation of arbitrary to absolute values. In addition we compared these data with one- and two-dimensional measurements under the microscope and an image-processing-system, respectively. |
| Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| huel71.pdf | 2,41 MB | Adobe PDF | Öffnen/Anzeigen |
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