Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-1951
Hülser, Dieter F.
|Title:||A rapid method for the determination of absolute particle sizes in a flow cytometer|
|metadata.ubs.publikation.source:||Schütt, Wolfgang (Hrsg.): Physical characterization of biological cells. Berlin : Verl. Gesundheit, 1991. - ISBN 3-333-00632-4, S. 131-146|
|Abstract:||The most precise determination of a cell-size distribution can be expected by registering the signal of a three-dimensional size (particle volume). This is best performed in a flow cytometer with an integrated volume measurement according to the Coulter-principle.This electrical measurement is very fast and is more precise than the forward-scatter method which is used alternativly in flow cytometry. The mean size of particles which has been determined by the Coulter-principle is mostly given by arbitrary units. We, therefore, present here a simple and fast calibration method which allows the transformation of arbitrary to absolute values. In addition we compared these data with one- and two-dimensional measurements under the microscope and an image-processing-system, respectively.|
|Appears in Collections:||04 Fakultät Energie-, Verfahrens- und Biotechnik|
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