Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-1952
Authors: Hülser, Dieter F.
Paschke, Dietmar
Greule, Joachim
Title: Gap junctions: correlated electrophysiological recordings and ultrastructural analysis by fast freezing and freeze-fracturing
Issue Date: 1989
metadata.ubs.publikation.typ: Buchbeitrag
metadata.ubs.publikation.source: Plattner, Helmut (Hrsg.): Electron microscopy of subcellular dynamics. Boca Raton : CRC Pr., 1989. - ISBN 0-8493-6079-X, S. 33-49
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-68780
http://elib.uni-stuttgart.de/handle/11682/1969
http://dx.doi.org/10.18419/opus-1952
Abstract: The effect of glutardialdehyde on the dynamic organization of gap junctions cannot only be seen by electrophysiological measurements where the uncoupling of cells occurs within 3 min, but also by freeze fracturing the cells. Gap junctions from unfixed cells rapidly frozen by dipping into liquid propane appear polymorphic; loosely packed and clustered plaques are found, as well as tightly packed aggregates, which are mainly found in fixed preparations. Whether these different structures correspond with different functional states, or whether they depend on the local configuration of the contacting membranes is difficult to decide. The presented results, however, support the idea of active (coupling competent) gap junctions with loosely packed channels and nonactive (permanently closed) gap junctions where the channels are tightly packed.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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