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Authors: Kremp, Anette
Schliephacke, Martin
Kull, Ulrich
Schmid, Hans-Peter
Title: Prosomes exist in plant cells too
Issue Date: 1986 Zeitschriftenartikel Experimental cell research 166 (1986), S. 553-557. URL
Abstract: A 19S particle was purified from tobacco (Nicotiana rustica) leaf cells. Its density was determined as 1296 g/cm3 in Cs2SO4-DMSO gradients, indicating the presence of RNA and protein. Polyacrylamide gel electrophoresis (PAGE) revealed eight distinct proteins in the range of 20-30 kD and RNA in the range of 70-80 nucleotides. Electron microscopic examination showed the same raspberry-shaped structure with a central depression as described for prosomes. We conclude that tobacco 19S particles represent small cytoplasmic complexes, possessing biochemical and structural characteristics similar to the hitherto known prosomes of animal cells.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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