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http://dx.doi.org/10.18419/opus-2348
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DC Element | Wert | Sprache |
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dc.contributor.author | Feuer, Ronny | de |
dc.contributor.author | Gottlieb, Katrin | de |
dc.contributor.author | Viertel, Gero | de |
dc.contributor.author | Klotz, Johannes | de |
dc.contributor.author | Schober, Steffen | de |
dc.contributor.author | Bossert, Martin | de |
dc.contributor.author | Sawodny, Oliver | de |
dc.contributor.author | Sprenger, Georg | de |
dc.contributor.author | Ederer, Michael | de |
dc.date.accessioned | 2015-01-23 | de |
dc.date.accessioned | 2016-03-31T07:54:52Z | - |
dc.date.available | 2015-01-23 | de |
dc.date.available | 2016-03-31T07:54:52Z | - |
dc.date.issued | 2012 | de |
dc.identifier.other | 425231348 | de |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-98271 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/2365 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-2348 | - |
dc.description.abstract | Bacterial strains that were genetically blocked in important metabolic pathways and grown under selective conditions underwent a process of adaptive evolution: certain pathways may have been deregulated and therefore allowed for the circumvention of the given block. A block of endogenous pyruvate synthesis from glycerol was realized by a knockout of pyruvate kinase and phosphoenolpyruvate carboxylase in E. coli. The resulting mutant strain was able to grow on a medium containing glycerol and lactate, which served as an exogenous pyruvate source. Heterologous expression of a pyruvate carboxylase gene from Corynebacterium glutamicum was used for anaplerosis of the TCA cycle. Selective conditions were controlled in a continuous culture with limited lactate feed and an excess of glycerol feed. After 200–300 generations pyruvate-prototrophic mutants were isolated. The genomic analysis of an evolved strain revealed that the genotypic basis for the regained pyruvate-prototrophy was not obvious. A constraint-based model of the metabolism was employed to compute all possible detours around the given metabolic block by solving a hierarchy of linear programming problems. The regulatory network was expected to be responsible for the adaptation process. Hence, a Boolean model of the transcription factor network was connected to the metabolic model. Our model analysis only showed a marginal impact of transcriptional control on the biomass yield on substrate which is a key variable in the selection process. In our experiment, microarray analysis confirmed that transcriptional control probably played a minor role in the deregulation of the alternative pathways for the circumvention of the block. | en |
dc.language.iso | en | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.subject.classification | Systembiologie | de |
dc.subject.ddc | 570 | de |
dc.subject.other | adaptive evolution , flux balance analysis | en |
dc.title | Model-based analysis of an adaptive evolution experiment with Escherichia coli in a pyruvate limited continuous culture with glycerol | en |
dc.type | article | de |
ubs.fakultaet | Fakultät Energie-, Verfahrens- und Biotechnik | de |
ubs.fakultaet | Fakultät Konstruktions-, Produktions- und Fahrzeugtechnik | de |
ubs.fakultaet | Fakultätsübergreifend / Sonstige Einrichtung | de |
ubs.institut | Institut für Mikrobiologie | de |
ubs.institut | Institut für Systemdynamik | de |
ubs.institut | Sonstige Einrichtung | de |
ubs.opusid | 9827 | de |
ubs.publikation.source | EURASIP journal on bioinformatics and systems biology (2012), 2012:14. URL http://dx.doi.org./10.1186/1687-4153-2012-14 | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Feuer2012.pdf | 756,53 kB | Adobe PDF | Öffnen/Anzeigen |
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